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EMERGENT LIFE SCIENCES RESEARCH - Vol 3, Issue 2, Published on 31, December 2017

Pages: 32-36
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Effect of different concentrations of sucrose on Alkaloids and Steroids production in vitro from Withania somnifera (L) Dunal (Ashwagandha)

Author: Ali Khalaf Hmood, Hussein Ali Salim

Category: Research Article


Present work has been conducted on Withania somnifera commonly known as Ashwagandha to study the influence of different sucrose concentrations on the production of high secondary metabolite compounds. With the addition of different sucrose concentrations 30, 60, 90 g / L to MS medium in in vitro conditions, production of secondary metabolite compounds increased. Ten compounds with different production rates were produced. Production rate of compound 17- hydroxy 27 deoxy withaferin, 27-hydroxy withanolide D and Withanone was maximum and significant under 30 g / L, 60 g / L, and 90 g / L sucrose treatment, respectively. However, MS medium containing 120 g / L sucrose led to burning of shoots and turned it in to brown.

Keywords: secondary metabolite compounds, sucrose