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  <Article>
    <Journal>
      <PublisherName>emergentresearch</PublisherName>
      <JournalTitle>Emergent Life Sciences Research</JournalTitle>
      <PISSN>2395-6658 (</PISSN>
      <EISSN>) 2395-664X (Print)</EISSN>
      <Volume-Issue/>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season/>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>-0001</Year>
        <Month>11</Month>
        <Day>30</Day>
      </PubDate>
      <ArticleType>Research Article</ArticleType>
      <ArticleTitle>Antimicrobial evaluation of Bovine Lactoferrin : A bioactive protein isolated from milk of crossbred and Indigenous cow breeds
</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>0</FirstPage>
      <LastPage>0</LastPage>
      <AuthorList>
        <Author>
          <FirstName>Mohammed Shaz</FirstName>
          <LastName>Murtuza</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>Lactoferrin (Lf) is a multifunctional iron binding glycoprotein, which exerts a broad-spectrum primary defense activity against bacteria, fungi, protozoa and viruses. The antibacterial activity of bovine lactoferrin isolated from the milk of crossbred and indigenous breeds of cattle was studied. The BLf protein was purified from the milk of HF crossbred and indigenous breeds like Sahiwal and Poda thurpu cows using the cation-exchange chromatography method and the antimicrobial activity was studied using broth microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC) of isolated BLf from the HF cross bred was found at 8000 μg/mL against S. aureus and that of Sahiwal and isolated BLf from Poda thurpu breed were 4000 μg/mL. The MIC against Bacillus spp. was at 4000 μg/mL for all the experimental cow breeds. The MIC value of isolated BLf from Holstein-Friesian (HF) cross bred against E.coli and Proteus spp. was 4000 μg/mL. However, the MIC for isolated Bovine lactoferrin (BLf) from Sahiwal and Poda thurpu breeds against E.coli and Proteus spp. in the present study was at 2000 μg/mL. The zone of inhibition was observed at 2000 μg/mL concentration and a larger inhibition zone was observed at 4000μg/mL by the isolated BLf  from all the three experimental breeds of cows against the selected gram positive and gram negative bacteria. However, more potency was shown by the BLf isolated from the indigenous breed viz. Poda thurpu followed by Sahiwal.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Bovine lactoferrin, Poda thurpu, Fast Protein liquid chromatography, MIC, Zone of inhibition</Keywords>
      <URLs>
        <Abstract>https://www.emergentresearch.org/ubijournal-v1copy/journals/abstract.php?article_id=14035&amp;title=Antimicrobial evaluation of Bovine Lactoferrin : A bioactive protein isolated from milk of crossbred and Indigenous cow breeds</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References/>
      </References>
    </Journal>
  </Article>
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