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  <Article>
    <Journal>
      <PublisherName>emergentresearch</PublisherName>
      <JournalTitle>Emergent Life Sciences Research</JournalTitle>
      <PISSN>2395-6658 (</PISSN>
      <EISSN>) 2395-664X (Print)</EISSN>
      <Volume-Issue/>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season/>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>-0001</Year>
        <Month>11</Month>
        <Day>30</Day>
      </PubDate>
      <ArticleType>Research Article</ArticleType>
      <ArticleTitle>Microsatellites based Parental Polymorphism Survey for Drought Tolerance in Rice (Oryza sativa L.) between the parental genotypes HUR-1309 and CR Dhan 801</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>0</FirstPage>
      <LastPage>0</LastPage>
      <AuthorList>
        <Author>
          <FirstName>PRASANTA KUMAR</FirstName>
          <LastName>MAJHI</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>The present investigation was conducted during Rabi 2018-19 and the parental polymorphism work was conducted at Molecular Drought Breeding Laboratory, Department of Genetics and Plant Breeding and Central Laboratory, Institute of Agricultural Sciences, BHU; and at Crop Improvement Division, ICAR-NRRI, Cuttack. The study was aimed to identify the polymorphic markers set for allelic variation between both parents, to study their distribution across the chromosomes and identify repeat motif. The polymorphic markers will be helpful to introgress the drought tolerant yield QTLs (qDTY) from the donor CR Dhan 801 into the background of popular high yielding variety HUR-1309 through marker assisted backcross breeding programme. The standard procedure of genomic DNA isolation, polymerase chain reaction and agarose gel electrophoresis were followed. Total 510 microsatellites were used for evaluation of the parental polymorphism and 90 markers showed polymorphism among the parents and the rest 420 markers are monomorphic for the studied QTLs (qDTY1.1, qDTY2.1 and qDTY3.1). The parental polymorphism in the present study ranged between 11.43% to 30.61%. Considering the 12 chromosomes of rice, the average polymorphism was observed 17.65%. Out of 90 polymorphic markers, 59 were di nucleotide repeats, 24 were trinucleotide repeats and 4 were tetra nucleotide repeats. The dinucleotides were highly present on chromosome 1 (12), 2 (9) and 3 (9) and repeated 12, 9 and 9 times respectively. The markers which are found to be polymorphic can be used in the marker-assisted backcross breeding (MABB) programme for grain yield under drought.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Drought tolerance,Marker-assisted backcross breeding,Microsatellites,Parental polymorphism,Rice</Keywords>
      <URLs>
        <Abstract>https://www.emergentresearch.org/ubijournal-v1copy/journals/abstract.php?article_id=14266&amp;title=Microsatellites based Parental Polymorphism Survey for Drought Tolerance in Rice (Oryza sativa L.) between the parental genotypes HUR-1309 and CR Dhan 801</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References/>
      </References>
    </Journal>
  </Article>
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